AEBSF hydrochloride is a specific irreversible serine protease inhibitor. AEBSF inhibits the rat embryo implantation in vivo and interferes with cell adhesion in vitro. Both AEBSF and PMSF are sulfonyl fluorides and are sulfonylating agents. Sulfonyl fluorides act by reacting with the hydroxy group of the active site serine residue to form a sulfonyl enzyme derivative. This derivative may be stable for long periods of time except at high pH.
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